Microarray Biochip Chemiluminescence

Immunoassay Detection Platform

This system utilizes a hard substrate biochip detection platform for easy and accurate quantitative detection of markers in human body. Multiple indexes were arranged on a chip in the form of microarray. Each index was tested four times in parallel, and each protein chip had negative and positive quality control. The platform is applicable to a variety of reaction principles such as double antigen sandwich method, indirect method, capture method and double antibody sandwich. The marker is horseradish peroxidase (HRP), which catalyzes luminol luminescence. The highly sensitive integrated photoelectric sensor can read all dot matrix optical signals of the whole chip at one time, so as to realize the perfect combination of high sensitivity of chemiluminescence and high throughput of biochip.

Platform Advantages

【High Throughput】 Integration of up to 56 lattices, each providing effective information;

【High Detection rate】 Achievements of the "863 Program", optimized combination of indicators and collaborative judgment to reduce missed diagnosis and misdiagnosis;

【High Accuracy】 Mainstream chemiluminescence immunoassay technology, ensuring high sensitivity, wide linearity range, and excellent repeatability;

【High Efficiency】 Less blood volume required; one chip is enough to complete the testing of all combinations of indicators, saving testing time;

【Automatic】 Automatic instrument, no manual operation required, avoiding human errors;

【Low Cost】 Integrate testing items into one chip to form a "lab on chip", which greatly reduces the cost.

Video Presentation

Source of Technology

Fig. 1: Document of the Ministry of Science and Technology during the 11^th Five-Year Plan 863 Program (No. 2007AA02Z499: Clinical Research on Optimized Combination of Tumor Markers and Research and Development of Biochip)

Fig. 2: Third Prize of Jiangsu Science and Technology Award (Project: Key Technology and Application of Microarray Chemiluminescence Biochip for Medical Diagnosis)